Capillary Electrophoresis

Capillary Electrophoresis

  • Fanavaran Nano Meghyas Co
  • Isolation and chromatography

    1. Certificate of Nanotechnology

    2. 2020/11/01
    3. 2023/11/01

    1. NanoScale Certification

    2. 2020/11/01
    3. 2023/11/01
Introduction Application Specification Advantage Of Using Nanotechnology Manual And Maintenance Safety And Package
Capillary electrophoresis is an analytical technique in which by applying an electric field, ions are separated based on their migration velocity in a capillary tube. The migration velocity depends on the charge of molecule, viscosity, and radius of atom. Typically, a capillary electrophoresis system consists of high-voltage power supply, injection system, capillary tube made of fused silica coated with polyimide, temperature controller, and detector. Each side of the high voltage power supply is connected to an electrode. These electrodes generate an electric field which helps the sample to move from anode towards the cathode through the capillary tube. Each side of the capillary tube is dipped in a vial containing electrolyte solution and an electrode. The separation process begins by replacing the sample vial with one of the buffer vials (usually anode) and applying an electric field. Detection is usually conducted through a small window at the end of the capillary tube near the cathode through passing UV-VIS light from the sample and measuring the absorption. 

Capillary electrophoresis has a wide range of applications in different industries. Some of its main applications are separation of compounds such as:

  • amino acids, chiral, vitamins, proteins, peptides, mineral ions, organic acids, carbohydrates, and specially measurement and characterization of nanomaterials.
Details of technical specifications are presented in the following Table.
 
Capillary electrophoresis is an analytical instrument which can be used for sizing and characterization of nanomaterials. Capillary electrophoresis is used to identify DNA sequencing, amino acids, chiral, vitamins, proteins, peptides, mineral ions, organic acids, carbohydrates, self-assembly, identification and separation of nanomaterials.
  • To initiate the device, first connect the power cord to the back of the device.
  • Make sure that the vials are put in their place properly.
  • When moving the outlet or inlet vials, make sure that there is no vial beneath them.
  • Avoid using the UV lamp without interruption for a long time.
  • To prevent capillary tube blockage, always use the filtered solutions.
  • For more details on how to use the device, refer to the device catalog and user guide.
  • During working with high voltages, make sure that the high voltage power cord is connected correctly, as well as the vials outer surfaces are not smeared with the solution.
  • Make sure that the vials outer surfaces are not smeared with the solution since it may cause the leakage of high voltages to the device body.
  • In case of noise emission due to the high voltage leakage, immediately switch off the high voltage and check the possible causes.
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